Five strains of Agrobacterium rhizogenes (LB510, LB509, YMB072001, A4T, and ATCC 15834) were tested to determine their effects on the induction of hairy root on leaf explants of Justicia gendarussa Burm. f.. Leaf explants were sterilized, and then infected within liquid MS medium + sucrose containing A. rhizogenes OD600 = 0.1, for 20 minutes while being shaken gently by hand. Teh explant was co-cultivated on Murashige and Skoog (MS) solid medium at 25�C for 48 hours. After 48 hours of co- cultivation, explants were transferred to MS solid medium with the addition of 250 mg/L cefotaxim, and repeated 1-2 times. Observations were carried out during 6 weeks to observe the specified parameters. Data were analized by Kruskal-Wallis Test and followed by Mann-Whitney Test. All strains of A. rhizogenes were used in this study were able to produce hairy root. The treatment of LB510 and YMB072001 strain was the best strain in the hairy root induction of J. gendarussa Burm. f.. The infection frequency of LB510 strain was highest ( 100 persen ), longest hairy root (2.23 cm), and highest of hairy root wet weight (0.0243 g). The treatment of YMB072001 strain was the highest number of hairy roots (3.3) per explant and the highest number of hairy roots dry weight (0.0098 g).