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Publikasi Staf Biotek

PENGARUH IRRADIASI SINAR GAMMA PADA PERTUMBUHAN KALUS DAN TUNAS TANAMAN GANDUM (Triticum Aestivum L.)



This aim of this research was to determine lethal dosage 20 (LD20) and 50 (LD50) calli wheat Dewata varieties after irradiated by gamma rays. The successful of gamma irradiation rays is influenced by the sensitivity of plant genotype. The experiments were arranged factorial in Randomized Completely Design 1 x 4, with 3 replications. The first factor is wheat Dewata varieties. The second factor is the gamma irradiation dose of 0; 7.5; 15; 22.5 and 30Gy. The observed variables were calli growth and percentage growth shoots. The LD20 and LD50 were determined using Curve-Fit Analysis. The results showed that the dose of irradiation significantly affect wheat calli growth inhibition. The percentage of live shoots decreased with increase in irradiation dose. Survival decrease drastically even shoots do not grow at all and colored albino at a dose of 30Gy. Highest percentage of live shoots at a dose of 15Gy. Contained the highest percentage of calli growth at doses of 7.5Gy. The higher dose of irradiation is used, the percentage of shoots that grow less or lower regeneration. Increased genetic diversity found in wheat calli irradiation treatment between LD20 and LD50 is the dose of gamma rays irradisi 15 - 22.5Gy. The best model lethal dose for calli growth quadratic form with the equation LD20= 0.99Gy and LD50 = 6.41Gy. As for the percentage of calli were able to germinate and generations have grown calli equations based on the percentage of LD20 = 13,14Gy and LD50 = 24,00Gy Keywords: Wheat (Triticum aestivum L.), lethal dosage, Gamma irradiation.


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Judul Seri
Jurnal Ilmu Pertanian 18 (1): 63 - 71
No. Panggil
-
Penerbit : .,
Deskripsi Fisik
-
Bahasa
Indonesia
ISBN/ISSN
0126-4214
Klasifikasi
NONE
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subyek
Info Detil Spesifik
-
Pernyataan Tanggungjawab

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