Image of Medium Chain And Long Chain Alkanes Hydroxylaseproducing Whole Cell Biocatalyst From Marine Bacteria

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Medium Chain And Long Chain Alkanes Hydroxylaseproducing Whole Cell Biocatalyst From Marine Bacteria



Alkanes are major component of crude oil that could be hydrolyzed by the enzyme of alkane hydroxylase. There are three types of alkane hydroxylase based on the chain length of alkane such as short-chain length/SCL (C2-C4), medium-chain length/MCL (C5-C17), and long-chain length/LCL (C>18). The aims of this study were to characterize and identify alkanes-degrading bacteria from these bacteria. The 30 strains from marine were grown on MCL (Pentane-C5H12, Decane-C10H22, and Pentadecane-C15H32) and LCL (n-Paraffin-C12H19C17 and branch of PristaneC19H40). The study showed twenty-nine isolates have the ability to degrade alkanes compounds, whereas 14 isolates have grown ability on MCL and LCL medium, 11 isolates have the ability to grow on MCL and n-LCL, 3 isolates have the ability only to grow on MCL medium and 1 isolate has the ability only grow on n-LCL medium. The growth test result indicated that 29 isolates have medium-chain alkane monooxygenase and long-chain alkane hydroxylase. Based on 16S rDNA gene analysis, we obtained twenty nine of oil- degrading bacteria, namely alpha-proteobacteria (57 %), gamma-proteobacteria (30 %), Flavobacteria (7 %), Bacilli (3%) and Propionibacteriales (3 %). Gamma-Proteobacteria and alpha-proteobacteria which seems to play an important role in the alkane biodegradation.

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Judul Seri
Annales Bogorienses
No. Panggil
-
Penerbit Pusat Penelitian Bioteknologi LIPI. : .,
Deskripsi Fisik
Vol. 22 (1): 12-19.
Bahasa
English
ISBN/ISSN
-
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NONE
Tipe Isi
text
Tipe Media
other
Tipe Pembawa
online resource
Edisi
-
Subyek
Info Detil Spesifik
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Pernyataan Tanggungjawab

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