Image of Medium Optimization for Antimicrobial Production by Newly Screened Lactic Acid Bacteria

Publikasi Staf Biotek

Medium Optimization for Antimicrobial Production by Newly Screened Lactic Acid Bacteria



Lactic acid bacteria (LAB) are important for prevention of spoilage and pathogenic bacterial growth in foods due to their ability to generate antimicrobial substances. The objectives of this study were to screen LAB for antimicrobial activity and to optimize culture medium for antimicrobial production using Response Surface Methodology (RSM) with Central Composite Design (CCD). Optimization of antimicrobial production of selected LAB was conducted with different combinations of glucose, NaCl, inoculum, and temperature. Our experimental results show that from 129 LAB isolates, 55 show significant inhibition against Bacillus subtilis, Escherichia coli, Micrococcus luteus, Staphylococcus aureus, Aspergillus niger, and Candida albicans. No isolates inhibited the growth of Aspergillus flavus. Lactobacillus plantarum LIPI13-2-LAB011 was selected for further study on culture medium optimization to inhibit the growth of C. albicans. Statistical analysis reveals that the production of antimicrobial substances was significantly influenced by temperature, NaCl, and concentration of glucose. Furthermore, the optimum concentrations of glucose, concentration of inoculum, temperature, and NaCl were 1.63 %, 3.03%, 33.74°C, and 3.4%, respectively, with a maximum predicted inhibition index of 1.916, which increased 3.56-fold compared to that obtained in medium before optimization processes. The result was confirmed as when the optimum concentration of nutritions used, the inhibition index increased 3.12-fold.

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Judul Seri
Annales Bogorienses
No. Panggil
-
Penerbit Pusat Penelitian Bioteknologi LIPI. : Cibinong, Bogor.,
Deskripsi Fisik
Vol. 22 (1): 1-11.
Bahasa
English
ISBN/ISSN
-
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NONE
Tipe Isi
text
Tipe Media
other
Tipe Pembawa
online resource
Edisi
-
Subyek
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-
Pernyataan Tanggungjawab

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