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Publikasi Staf Biotek

Produksi beta-glukan Saccharomyces cerevisiae dalam media dengan sumber nitrogen berbeda pada air-lift fermentor: β-Glucan production of Saccharomyces cerevisiae in medium with different nitrogen sources in air-lift fermentor



β-Glucan is one of the most abundant polysaccharides in yeast Saccharomyces cerevisiae cell wall. The aim of this research is to explore an alternative nitrogen sources for β-glucan production. S. cerevisiae were grown in fermentation medium with different nitrogen sources. Peptone 2%, glutamic acid 0,5%, urea 0,2%, and dlammonium hydrogen phosphate (DAHP) 0.02% were used for nitrogen source in the medium. A two liter air-lift fermentor was used In the fermentation process for 84 hours (T '" 30°C. pH 7. and 1.5 vvm for the aeration). During the fermentation, optical density, extraction of β-glucan, glucose and protein in hydrolisate cultured were determined. β-glucan production level is similar with the growth rate of yeast and followed by decreasing glucose and protein content in hydrolysis cultured. The highest and lowest β-glucan content were obtained from peptone (933.33 mg/l) and glutamic acid (633.33 mg/L) as a nitrogen source in cells cultured after fermentation completed respectively. Yeast cells cultured with urea and DAHP as a nitrogen source give the same content of β-glucan about 733.33 mglL. β-glucan concentration produced in medium with urea was a higher than that produced using glutamic acid and DAHP as a nitrogen source. The result indicated that urea can be used as an alternative nitrogen source for the production of β -glucan. Urea is easily available and cheaper than peptone, glutamic acid and DAHP.

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Judul Seri
Biodiversitas
No. Panggil
-
Penerbit : .,
Deskripsi Fisik
Volume 8 Nomor 2, Oktober 2007
Bahasa
Indonesia
ISBN/ISSN
ISSN 1412-033X
Klasifikasi
NONE
Tipe Isi
text
Tipe Media
-
Tipe Pembawa
online resource
Edisi
-
Subyek
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-
Pernyataan Tanggungjawab

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