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Publikasi Staf Biotek

Potency of Hibiscus (Hibiscus ros A-sinensis L.) as antioxidant to reduce Carbon tetra chloride compounds in red blood cells



Hibiscus (hibiscus rosa-sinensis L.) is a species of flowering plant that is easily found and used for traditional medicine. hibiscus flower decoction is used to treat various diseases, such as urinary tract infections, coughing, whooping cough, dysentery, bronchitis, tuberculosis and various degenerative diseases. These properties are related to the content of compounds as antioxidants such as anomuricin and polyphenols. Antioxidant compounds may reduce the risk due to free radical oxidation process to prevent degenerative diseases. hibiscus flowers were extracted by maceration method with ethanol and ethyl acetate solvents. The extract containing antioxidant of 10, 20, 30, and 40 ppm was tested against sheep red blood cell that induced with CCl4 as oxidant. Parameters measured were malondialdehyde (mDA) content and activity of superoxide dismutase (SOD) and catalase enzymes. Data was analyzed using Kruskal-Wallis test. Results showed that the highest reduction in mDA content (0.7679 nmol/ml) was obtained by the treatment of ethyl acetate extract at the concentration of 10 ppm. The highest activities of the SOD and catalase enzymes were 0.8874 U/ml and 460.655 u/ml respectively, obtained by the treatment of 40 ppm of the extract containing antioxidants. (sef, 12-2-2015)


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Judul Seri
Proceeding ASEAN COSAT 2014, 9th ASEAN Science and Technology Week (ASTW-9)
No. Panggil
-
Penerbit : Jakarta.,
Deskripsi Fisik
p. 29-36
Bahasa
English
ISBN/ISSN
ISBN 978-979-799-812
Klasifikasi
NONE
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subyek
Info Detil Spesifik
-
Pernyataan Tanggungjawab

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