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Publikasi Staf Biotek

Nitrogen Fixing Potential of Endophytic Bacteria Isolated from Aloe barbadensis and Aloe sp.



Aloe is a crassulacean acid metabolism (CAM) species that are known to live in extreme environment such as drought condition. Nitrogen fixation process influenced by the ability of plants to adapt in drought condition. Endophytic bacteria from Aloe and their ability for nitrogen fixation were little reported, but potential and its relationship between the ability for nitrogen fixing with resistance to drought conditions have been not reported. This research aimed study the endophytic bacteria from two varieties of aloe, namely Aloe barbadensis Miller and Aloe sp. in their ability conducting the nitrogen fixing process and its relationship with resistance to drought. Characterization of endophytic bacteria was carried out by morphological observation of colony, Gram staining and molecular identification. Screening of nitrogen fixing was done using Nitrogen-free semisolid Nfb malate medium. Endophytic bacteria from Aloe sp. more than A. barbadensis in their potency of nitrogen fixation which related with habitat where their planted. A total of 40 persen of the endophytic isolates from the leaves of the aloe var. A. barbadensis and 62.5 persen of isolates from var. Aloe sp. are known to have a better ability to fixing nitrogen than the others. Isolates A. barbadensis AB12 and Aloe sp. AS8 were the best isolates from each variety on ability to nitrogen fixation. Based on 16S rRNA gene analysis those two selected isolates belonged to Bacillus methalotropicus strain DA 16-5 Bacillus aryabatthai strain B8 W22.

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Judul Seri
Microbiology Indonesia
No. Panggil
-
Penerbit : .,
Deskripsi Fisik
Vol. 10 (2): 48-56.
Bahasa
English
ISBN/ISSN
1978-3477
Klasifikasi
NONE
Tipe Isi
text
Tipe Media
-
Tipe Pembawa
online resource
Edisi
-
Subyek
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Pernyataan Tanggungjawab

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