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Publikasi Staf Biotek

Induksi Tanaman Poliploid Talas (Colocasia esculenta L.) dengan Perlakuan Orizalin secara In Vitro: Induction of Taro (Colocasia esculenta L.) Polyploid by Oryzalin In Vitro Treatment



Genetic improvement of taro (Colocasia esculenta L.) is one of important research program to increase productivity and give better cultivation on the marginal land. Induction of polyploid plants is a method useful to increase genetic diversity. The aim of this research was to induce polyploid taro by oryzalin treatment. Polyploidy was induced from in vitro diploid taro ‘bentul’ using oryzalin at 7.5; 15; 30; 60 and 75 µM soaked for 3 days. Regenerated shoots were grown on MS medium containing 2 mg/l BAP, 1 mg/l thiamine and 2 mg/l adenine. Shoot growth was recorded four weeks (subculture-0), eight weeks (subculture-1) and twelve weeks (subculture-2) after treatments. The results showed that survival rate of treated shoots was 100%. Higher oryzalin concentration reduced the proliferation of shoots, petiole length, numbers of leaves as well as the numbers of roots. Ploidy levels analysis determined by flowcytometer for 122 plantlets were investigated. The results indicated that control shoots were diploid. All treated shoots were polyploids. Oryzalin at 60 µM gave 50% of tetraploid planlets, 30 µM of oryzalin gave 5.71% hexaploids, 60 µM oforyzalin gave 9.09% octaploids. All acclimatized plantlets gave 100% survival rate.

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Judul Seri
Jurnal Biologi Indonesia
No. Panggil
-
Penerbit : .,
Deskripsi Fisik
12 (2): 297-305.
Bahasa
Indonesia
ISBN/ISSN
-
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NONE
Tipe Isi
text
Tipe Media
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Tipe Pembawa
online resource
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Subyek
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