Image of Production of Mannanase from Bacillus Subtilis LBF-005 and Its Potential for Manno-oligosaccharides Production

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Production of Mannanase from Bacillus Subtilis LBF-005 and Its Potential for Manno-oligosaccharides Production



Endo- -1, 4-mannanase is the key enzymes for -1,4-linkages within the mannan backbone releasing manno-oligosaccharides (MOS). A marine bacterium of Bacillus subtilis LBF-005 was reported have ability to produce endo-type mannanase. The aims of this research were to compare commercial biomass Locust Bean Gum (LBG) and raw biomass contaning mannan as carbon source for mannanase production from Bacillus subtilis LBF-005, to analyze the optimum condition of mannanase production, and to find out the potential of the mannanase for MOS production. Bacillus subtilis LBF-005 was cultivated in Artificial Sea Water (ASW) medium contain NaCl and various mannan biomass as carbon source for mannanase production. The cells were grown in submerged fermentation. The maximum enzyme activity was obtained with porang potato as a substrate with concentration 1 persen, pH medium 8, and incubation temperature 50°C with an enzyme activity of 37.7 U/mL. The mainly MOS product released by crude mannanase produced by Bacillus subtilis LBF-005 were mannobiose (M2), mannotriose (M3), mannotetraose (M4), and mannopentaose (M5).

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Judul Seri
Proceedings of the 3rd International Symposium on Applied Chemistry 2017
No. Panggil
-
Penerbit AIP Publishing : .,
Deskripsi Fisik
Vol. 1904, Issue 1: 020022-1–020022-9.
Bahasa
English
ISBN/ISSN
978-0-7354-1594-2
Klasifikasi
NONE
Tipe Isi
text
Tipe Media
other
Tipe Pembawa
online resource
Edisi
-
Subyek
-
Info Detil Spesifik
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Pernyataan Tanggungjawab

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