Perpustakaan Bioteknologi
Lembaga Ilmu Pengetahuan Indonesia

Tandem Recombinant Plasmid Construction as Positive Control for PIK3CA H1047R Detection Based on SYBR Green I qPCR

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PIK3CA H1047R mutation is found in breast cancer in high frequency and its detection could be applied as prognosis and predictive factor for trastuzumab therapy. qPCR is one of the simplest and robust method for PIK3CA H1047R detection. Provision of positive control for PIK3CA H1047R detection based on qPCR will support data analysis efficiently and avoid false negative result. In this research, we constructed a tandem recombinant plasmid (pGEM-tandPIK3CA) as positive control for Tm Shift SYBR Green I qPCR-based of PIK3CA H1047R detection system by ligating wild-type and PIK3CA H1047R fragments tandemly into pGEM-T Easy. The tandem plasmid was confirmed by restriction, DNA sequencing and qPCR. As a result, pGEMtandPIK3CA has been successfully constructed and confirmed. Statistical analysis shows high repeatability and reproducibility with % CV of
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Judul Seri

Pak. J. Biotechnol.

No. Panggil

-

Penerbit

: .,

Deskripsi Fisik

Vol. 15 (3): 735-742.

Bahasa

English

ISBN/ISSN

1812-1837

Klasifikasi

NONE

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text

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other

Tipe Pembawa

online resource

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-

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This software and this template are released Under GNU GPL License Version 3.